Pre-Column Chelation Liquid Chromatographic Determination of Trace Cu, Zn, Ni, Co, Fe, Cd and Hg in Fish Samples


ALPDOĞAN G., Zor S. D., SAN N.

CURRENT ANALYTICAL CHEMISTRY, vol.13, no.4, pp.291-298, 2017 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 13 Issue: 4
  • Publication Date: 2017
  • Doi Number: 10.2174/1573411012666160614083418
  • Journal Name: CURRENT ANALYTICAL CHEMISTRY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.291-298
  • Yıldız Technical University Affiliated: Yes

Abstract

Background: This study describes a simple and rapid analytical method for the simultaneous determination of trace amounts of copper(II), zinc(II), nickel(II), cobalt(II), iron(II), cadmium(II) and mercury(II) in fish samples by the reversed-phase highperformance liquid chromatography (RP-HPLC) with di-2-pyridyl ketone benzoylhydrazone (DPKBH) as pre-column derivatization reagent. 

Methods: Seven metal chelates were successfully separated with methanol: acetonitrile: tetrabutyl ammonium bromide (1.0 mM): water mixture containing ammonium acetate buffer (pH 6.0) and DPKBH (1.0 mM) (40:24:1:30:5, v/v/v/v/v) as mobile phase on a C-18 column at a flow rate 1.0 mL min(-1) at 370 nm. 

Results: Under the optimum conditions, reproducibilities of analyte retention times (relative standard deviation (RSD) <= 0.04%) and peak areas (RSD <= 1.5%) were found to be good with the lineraties R2 >= 0.9995. The validation of the method was performed by the analysis of certified reference materials. 

Conclusion: The method developed was applied for the determination of Cu(II), Zn(II), Ni(II), Co(II), Fe(II), Cd(II) and Hg(II) in fish samples.

Background: This study describes a simple and rapid analytical method for the simultaneous determination of trace amounts of copper(II), zinc(II), nickel(II), cobalt(II), iron(II), cadmium(II) and mercury(II) in fish samples by the reversed-phase highperformance liquid chromatography (RP-HPLC) with di-2-pyridyl ketone benzoylhydrazone (DPKBH) as pre-column derivatization reagent.