Effect of different plant growth regulator on in vitro propagation of endangered plant; yellow tomato (Lycopersicon esculentum Mill.)

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Jawad Z. A., Türker M., Özdemir F. A.

International Journal of Agriculture Forestry and Life Sciences, vol.4, no.1, pp.92-98, 2020 (Peer-Reviewed Journal)


In the present study, a convinient procedure for in vitro propagation of yellow tomato plant, (Lycopersicon esculentum Mill.) under the risk of extinction, was developed. Shoot tip, hypocotyl, cotyledon, leaf, node and internode were excised from sprout seedlings and used for explant sources. In vitro adventitious shoot regeneration was achieved in MS medium supported with the particular concentrations and combinations of plant growth regulators (PGRs) via direct organogenesis. The most successful (100%) adventitious shoot regeneration was provided from node and shoot tip explants in MS medium supported with the particular concentrations and combinationss of 6-Benzylaminopruine (BAP) and Naphtalane acetic acid (NAA). The highest root formation (100%) was achieved on regenerated adventitious shoot in MS medium supported with Indole-3-acetic acid (IAA) and Indole-3-butyric acid (IBA). The whole seedlings regenerated in vitro were adapted to soil and acclimatized in field.