A rapid spectrophotometric method to resolve a binary mixture of food colorants (riboflavine and sunset yellow)


TURKISH JOURNAL OF CHEMISTRY, vol.28, no.3, pp.325-333, 2004 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 28 Issue: 3
  • Publication Date: 2004
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.325-333
  • Yıldız Technical University Affiliated: Yes


A very simple and rapid spectrophotometric method using measurements at zero-crossing wavelength is described for resolving binary mixtures of riboflavine (E-101) and sunset yellow (E-110) in a powder drink. The assay procedure for E-110 and E-101 involves the extraction of the colorants from the powder drink with pH 4.5 acetate buffer, filtration, appropriate dilution, and measurement of the first derivative absorbance values (denoted as D-1). Calibration graphs are linear tip to 25.0 mug.mL(-1) for E-101 and 40.0 mug.mL(-1) for E-110. The sensitivity and reproducibility of the method were determined by using the synthetic mixtures in different ratios prepared by us, and the proposed method was applied to a commercially available powder drink. The relative standard deviations obtained are 1.39% and 1.15% and the average percentage recoveries are 101.0% and 102.8% for E-101 and E-110, respectively.