Structural and physicochemical characterisation and antioxidant activity of an α-D-glucan produced by sourdough isolate Weissella cibaria MED17

Aburas H., İspirli H., Taylan O., Yilmaz M. T., DERTLİ E.

International Journal of Biological Macromolecules, vol.161, pp.648-655, 2020 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 161
  • Publication Date: 2020
  • Doi Number: 10.1016/j.ijbiomac.2020.06.030
  • Journal Name: International Journal of Biological Macromolecules
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, EMBASE, Food Science & Technology Abstracts, INSPEC, MEDLINE, Veterinary Science Database
  • Page Numbers: pp.648-655
  • Keywords: Exopolysaccharides, Antioxidant polysaccharides, Functional roles, GLUTEN-FREE, LACTOBACILLUS, EXOPOLYSACCHARIDES, DEXTRAN, GLUCANSUCRASE, BIOACTIVITY, ENZYMES, WHEAT
  • Yıldız Technical University Affiliated: Yes

Abstract

An exopolysaccharide (EPS) producer slimy-mucoid type colony was isolated from sourdough and identified as Weissella ciboria MED17. The H-1 and C-13 NMR spectra of EPS MED17 demonstrated that this EPS was a dextran type glucan ((1 -> 6)-linked alpha-D-glucose core structure) containing (1 -> 3)-linked alpha-D-glucose branches and proportion of (1 -> 6)-linked alpha-D-glucose units to (1 -> 3)-linked alpha-D-glucose units was 943:5.7%. The MR analysis also confirmed the (1 -> 6)-linked alpha-D-glucose linkage. A high level of thermal stability was observed for glucan MED17 as no degradation up to 300 degrees C was observed by TGA and DSC analysis. The XRD analysis of glucan MED17 showed its semi- crystalline nature and its compact sheet-like morphology was observed by SEM analysis. Finally, antioxidant characteristics of glucan MED17 were determined by ABTS and DPPH radical scavenging activity tests that revealed a moderate antioxidant activity of glucan MED17. These findings show potential techno-functional characteristics of glucan MED17. (C) 2020 Elsevier B.V. All rights reserved.