A rapid, sensitive and accurate determination of cobalamin with double monitoring system: HPLC-UV and HPLC-ICP-OES


Bodur S., Erarpat S., Balçık U., Bakırdere S.

Food Chemistry, vol.340, 2021 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 340
  • Publication Date: 2021
  • Doi Number: 10.1016/j.foodchem.2020.127945
  • Journal Name: Food Chemistry
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, PASCAL, Aerospace Database, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, Chemical Abstracts Core, Chimica, Communication Abstracts, Compendex, EMBASE, Food Science & Technology Abstracts, MEDLINE, Metadex, Veterinary Science Database, Civil Engineering Abstracts
  • Keywords: Cobalamin, Cobalt, High-performance liquid chromatography, Inductively coupled plasma-optic emission spectrometry, Kefir, PERFORMANCE LIQUID-CHROMATOGRAPHY, DAIRY-PRODUCTS, VITAMIN-B-12, EXTRACTION, MICROEXTRACTION, KEFIR, SAMPLES, NICKEL, MILK
  • Yıldız Technical University Affiliated: Yes

Abstract

© 2020 Elsevier LtdThis study proposed a novel analytical method for the separation and determination of cobalamin and cobalt in kefir samples by high performance liquid chromatography-inductively coupled plasma-optical emission spectrometry (HPLC-ICP-OES) in addition to determination of cobalamin in HPLC system. Chromatographic parameters such as column type, buffer solution, mobile phase flow rate and sample injection volume were individually studied and optimized. In addition, cobalamin was simultaneously determined by high performance liquid chromatography with ultraviolet detection (HPLC-UV). LOD values of cobalt in cobalamin and cobalt for HPLC-ICP-OES system were calculated as 0.07 mg/kg (as Co) and 0.06 mg/kg, respectively. Recovery studies were conducted to evaluate the accuracy/applicability of the method. Recovery results for cobalt in cobalamin and cobalt detected by the HPLC-ICP-OES system were calculated in the range of 87.4–100.1 and 98.8–115.0%, respectively while recovery results for cobalamin were found to be between 89.2 and 98.3% for HPLC-UV system.