Detection of cystine and cysteine in wheat flour using a robust LC-MS/MS method

DOGAN C. E., ÇEBİ N., Develioglu A., OLGUN E. O., SAĞDIÇ O.

JOURNAL OF CEREAL SCIENCE, cilt.84, ss.49-54, 2018 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 84
  • Basım Tarihi: 2018
  • Doi Numarası: 10.1016/j.jcs.2018.09.015
  • Dergi Adı: JOURNAL OF CEREAL SCIENCE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.49-54
  • Anahtar Kelimeler: Cystine, Cysteine, Bakery, Chromatography, Tandem mass, Flour, Wheat, SIMULTANEOUS QUANTIFICATION
  • Yıldız Teknik Üniversitesi Adresli: Evet

Özet

An effective and simultaneous liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for the first time, with the aim of quantification of cysteine and cystine in wheat flour. Developed LC-MS/MS method showed good linearity (R-2 = 0.99) with very low LOD and LOQ values as 2.0 mu g/kg and 7.0 mu g/kg for cystine, 3.0 mu g/kg and 8.0 mu g/kg for cysteine, respectively. Favorable repeatability and reproducibility were achieved. Developed highly sensitive LC-MS/MS method was successfully applied for the detection and quantification of cystine and cysteine in wheat flour samples. Cystine and cysteine contents were determined as quite similar and appropriate for 25 types of wheat flour. In addition, method has great capability for quantification of additional cysteine and cystine in the wheat flour since quite favorable recovery values were obtained.