A rapid ATR-FTIR spectroscopic method for classification of gelatin gummy candies in relation to the gelatin source
FOOD CHEMISTRY, cilt.277, ss.373-381, 2019 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 277
- Basım Tarihi: 2019
- Doi Numarası: 10.1016/j.foodchem.2018.10.125
- Dergi Adı: FOOD CHEMISTRY
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.373-381
- Anahtar Kelimeler: FTIR spectroscopy, Gelatin, Chemometrics, Source determination, Gummy candy, Halal food, INFRARED-SPECTROSCOPY, PORCINE GELATINS, MARKER PEPTIDES, BOVINE, ADULTERATION, DIFFERENTIATION, QUANTIFICATION, CHALLENGES, PREDICTION, HONEYS
- Yıldız Teknik Üniversitesi Adresli: Evet
Özet
Gelatin is widely used in gummy candies because of its unique functional properties. Generally, porcine and bovine gelatins are used in the food industry. FTIR-ATR combined with chemometrics analysis such as hierarchical cluster analysis (HCA) (OPUS Version 7.2 software), principal component analysis (PCA) (OPUS Version 7.2 software) and partial least squares-discriminant analysis (PLS-DA) (Matlab R2017b) were used for classification and discrimination of gelatin gummy candies related to their gelatin source. The spectral region between 1734 and 1528 cm(-1) was selected for chemometric analysis. The potential of FTIR spectroscopy for determination of bovine and porcine source in gummy candies was examined and validated by a real-time polymerase chain reaction (PCR) method. Twenty commercial samples were tested by developed ATR-FTIR methodology and RT-PCR technique, mutually confirming and supporting results were obtained. Gummy candies were classified and discriminated in relation to the bovine or porcine source of gelatin with 100% success without any sample preparation using FTIR-ATR technique.