Effect of hydrolysis products and Mg2+, Mn2+ and Ca2+ ions on whey lactose hydrolysis and beta-galactosidase stability

Demirhan E., Apar D. K., ÖZBEK B.

JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, cilt.83, sa.5, ss.625-636, 2008 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 83 Sayı: 5
  • Basım Tarihi: 2008
  • Doi Numarası: 10.1002/jctb.1840
  • Dergi Adı: JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.625-636
  • Anahtar Kelimeler: whey lactose hydrolysis, beta-galactosidase, hydrolysate, glucose, galactose, magnesium, manganese, calcium, modelling, KLUYVEROMYCES-FRAGILIS, STARCH HYDROLYSIS, ESCHERICHIA-COLI, KINETIC-MODEL, INACTIVATION, INHIBITION, BIOREACTOR, SUPPORTS, PERMEATE, REACTOR
  • Yıldız Teknik Üniversitesi Adresli: Evet

Özet

BACKGROUND: Enzyme inhibition is one of the constraints of reactions catalysed by enzymes, and information is required on the inhibition mechanisms that affect the process yield. Therefore the aim of the present study was to investigate the effect of hydrolysis products and ions on the hydrolysis of lactose recovered from whey and enzyme inactivation during the reaction. The experiments were carried out in 250 mL of 25 mmol L-1 phosphate buffer solution using beta-galactosidase from Kluyveromyces marxianus lactis in a batch reactor system.